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M9490575.TXT
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1994-09-24
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Document 0575
DOCN M9490575
TI [Expression of the gene for the gp46 surface glycoprotein from the human
T-cell leukemia virus type I (HTLV-I) in bacteria]
DT 9411
AU Sankov MN; Bobkov AF; Garaev MM
SO Mol Gen Mikrobiol Virusol. 1994 May-Jun;(3):23-5. Unique Identifier :
AIDSLINE MED/94344161
AB A set of recombinant plasmids containing different fragments of HTLV-I
env gene has been constructed on the basis of pUR290-pUR292 vectors. The
hybrid proteins containing different fragments of ENV predecessor in the
C-terminal of beta-galactosidase differed in stability in Escherichia
coli cells. The presence of N-terminal of ENV predecessor in recombinant
proteins considerably decreases their resistance to proteases of the
bacterial cell. Elimination of this fragment led to obtaining of the
recombinant plasmid pESG coding for the high level of synthesis of the
env-specific hybrid polypeptide (up to 30% of the total cellular
protein). This 134 Kda protein is able to interact efficiently with the
HTLV-I positive sera and may be used in the diagnostic test-systems for
identification of the HTLV-I infected patients.
DE beta-Galactosidase/GENETICS Cloning, Molecular English Abstract
Escherichia coli/GENETICS Gene Expression Gene Products, env/*GENETICS
HTLV-I Antigens/*GENETICS Plasmids Retroviridae Proteins,
Oncogenic/*GENETICS JOURNAL ARTICLE
SOURCE: National Library of Medicine. NOTICE: This material may be
protected by Copyright Law (Title 17, U.S.Code).